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multi-beam scanning electron microscope  (Carl Zeiss)


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    Carl Zeiss multi-beam scanning electron microscope
    Multi Beam Scanning Electron Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multi-beam scanning electron microscope/product/Carl Zeiss
    Average 90 stars, based on 1 article reviews
    multi-beam scanning electron microscope - by Bioz Stars, 2026-06
    90/100 stars

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    (a) Non-lesional brain tissue (purple) was obtained from the left middle temporal gyrus (red) of a 45-year-old female undergoing surgery for hippocampal sclerosis, drop-fixed in glutaraldehyde/paraformaldehyde fixative, stained with osmium tetroxide, and embedded in resin . The tissue was subsequently scanned with a high-resolution <t>multi-beam</t> <t>scanning</t> <t>electron</t> <t>microscope.</t> (b) The 200×200×112 μ m 3 subset of the EM volume corresponding to subcortical white matter (green rectangle in the right panel of (a)) was segmented using 2d and 3d U-Nets. (c) The segmented intra-axonal space of myelinated axons longer than 33.5 μ m were aligned along the z-axis, resulting in 76 axons ranging from 33.5 to 189.3 μ m long. The figure (a) is adapted from with permission from bioRxiv.
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    (a) Non-lesional brain tissue (purple) was obtained from the left middle temporal gyrus (red) of a 45-year-old female undergoing surgery for hippocampal sclerosis, drop-fixed in glutaraldehyde/paraformaldehyde fixative, stained with osmium tetroxide, and embedded in resin . The tissue was subsequently scanned with a high-resolution <t>multi-beam</t> <t>scanning</t> <t>electron</t> <t>microscope.</t> (b) The 200×200×112 μ m 3 subset of the EM volume corresponding to subcortical white matter (green rectangle in the right panel of (a)) was segmented using 2d and 3d U-Nets. (c) The segmented intra-axonal space of myelinated axons longer than 33.5 μ m were aligned along the z-axis, resulting in 76 axons ranging from 33.5 to 189.3 μ m long. The figure (a) is adapted from with permission from bioRxiv.
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    (a) Non-lesional brain tissue (purple) was obtained from the left middle temporal gyrus (red) of a 45-year-old female undergoing surgery for hippocampal sclerosis, drop-fixed in glutaraldehyde/paraformaldehyde fixative, stained with osmium tetroxide, and embedded in resin . The tissue was subsequently scanned with a high-resolution multi-beam scanning electron microscope. (b) The 200×200×112 μ m 3 subset of the EM volume corresponding to subcortical white matter (green rectangle in the right panel of (a)) was segmented using 2d and 3d U-Nets. (c) The segmented intra-axonal space of myelinated axons longer than 33.5 μ m were aligned along the z-axis, resulting in 76 axons ranging from 33.5 to 189.3 μ m long. The figure (a) is adapted from with permission from bioRxiv.

    Journal: bioRxiv

    Article Title: The influence of axonal beading and undulation on axonal diameter mapping

    doi: 10.1101/2023.04.19.537494

    Figure Lengend Snippet: (a) Non-lesional brain tissue (purple) was obtained from the left middle temporal gyrus (red) of a 45-year-old female undergoing surgery for hippocampal sclerosis, drop-fixed in glutaraldehyde/paraformaldehyde fixative, stained with osmium tetroxide, and embedded in resin . The tissue was subsequently scanned with a high-resolution multi-beam scanning electron microscope. (b) The 200×200×112 μ m 3 subset of the EM volume corresponding to subcortical white matter (green rectangle in the right panel of (a)) was segmented using 2d and 3d U-Nets. (c) The segmented intra-axonal space of myelinated axons longer than 33.5 μ m were aligned along the z-axis, resulting in 76 axons ranging from 33.5 to 189.3 μ m long. The figure (a) is adapted from with permission from bioRxiv.

    Article Snippet: The tissue sample was trimmed to 2×3×0.2 mm 3 and scanned with a multi-beam serial-section scanning electron microscope (Sigma, Carl Zeiss) in 4×4 nm 2 pixels and 33 nm slice thickness.

    Techniques: Staining, Microscopy